Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.119518 |
Chromosome: | chromosome 12 |
Location: | 8011191 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre12.g551850 | NFR1 | (1 of 1) 1.5.1.30 - Flavin reductase (NADPH) / Riboflavine mononucleotide reductase; NADH-dependent flavin reductase/cytochrome P450, CYP102 superfamily | 3'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCAGGCCAACCAGGGAATAGCAATTTA |
Internal bar code: | CGAGATAGCCATTGTGGGGCTA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 10 |
LEAP-Seq percent confirming: | 87.6195 |
LEAP-Seq n confirming: | 1741 |
LEAP-Seq n nonconfirming: | 246 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGCAGCCGACTTCTACTACC |
Suggested primer 2: | GGGAACATGTACGGGAGCTA |