Insertion junction: LMJ.RY0402.119518_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.119518
Chromosome:chromosome 12
Location:8011191
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g551850 NFR1 (1 of 1) 1.5.1.30 - Flavin reductase (NADPH) / Riboflavine mononucleotide reductase; NADH-dependent flavin reductase/cytochrome P450, CYP102 superfamily 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAGCAGGCCAACCAGGGAATAGCAATTTA
Internal bar code:CGAGATAGCCATTGTGGGGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:10
LEAP-Seq percent confirming:87.6195
LEAP-Seq n confirming:1741
LEAP-Seq n nonconfirming:246
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGCAGCCGACTTCTACTACC
Suggested primer 2:GGGAACATGTACGGGAGCTA