Insertion junction: LMJ.RY0402.119526_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.119526
Chromosome:chromosome 7
Location:2937577
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g332750 PSL2 Protein of unknown function; (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGTTGCAAAGGGAAACAACAAGTGCGCG
Internal bar code:TCGAGCTAGGGCCACGGTCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:228
LEAP-Seq percent confirming:89.1791
LEAP-Seq n confirming:956
LEAP-Seq n nonconfirming:116
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCATTTCGACTTGTTGAG
Suggested primer 2:AGGTGTCCAGCTGCATCTTT