Insertion junction: LMJ.RY0402.119547_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.119547
Chromosome:chromosome 14
Location:761219
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre14.g612850 (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCCGGAGGAGGCGGTGGAGGTGGTGGAG
Internal bar code:CTGAAGCCTCGACAAGCACGC

Confirmation - LEAP-Seq

LEAP-Seq distance:431
LEAP-Seq percent confirming:53.3157
LEAP-Seq n confirming:201
LEAP-Seq n nonconfirming:176
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GCTACGACAGGAGGTCAAGC
Suggested primer 2:CAGGCTCTTTATGACGGCTC