Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.119547 |
Chromosome: | chromosome 14 |
Location: | 761219 |
Confidence (%): | 58 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre14.g612850 | (1 of 93) 6.3.2.19 - Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCGGAGGAGGCGGTGGAGGTGGTGGAG |
Internal bar code: | CTGAAGCCTCGACAAGCACGC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 431 |
LEAP-Seq percent confirming: | 53.3157 |
LEAP-Seq n confirming: | 201 |
LEAP-Seq n nonconfirming: | 176 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCTACGACAGGAGGTCAAGC |
Suggested primer 2: | CAGGCTCTTTATGACGGCTC |