| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119552 |
| Chromosome: | chromosome 16 |
| Location: | 4435162 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g671000 | NDA5 | Type-II NADH dehydrogenase; (1 of 1) K17872 - NADH:ubiquinone reductase (non-electrogenic) (ndh2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGTGGTGGTGGGCGCGGGCTACGCGGG |
| Internal bar code: | AGGGAAACGCCATGGCGAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 104 |
| LEAP-Seq percent confirming: | 37.4913 |
| LEAP-Seq n confirming: | 1079 |
| LEAP-Seq n nonconfirming: | 1799 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCTCTGTCCTGCTGCCT |
| Suggested primer 2: | CGTCGGCACTTCACTTCATA |