| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.119591 |
| Chromosome: | chromosome 3 |
| Location: | 1397416 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g150900 | (1 of 1) IPR011146//IPR016030//IPR027417 - HIT-like domain // Adenosylcobalamin biosynthesis, ATP:cob(I)alamin adenosyltransferase-like // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGAACAAACGAATGGCATGCACTTGTTCA |
| Internal bar code: | TTATGGAGCTACGATGTAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 614 |
| LEAP-Seq percent confirming: | 99.4407 |
| LEAP-Seq n confirming: | 8179 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACCCTGTCGTGCGTTAT |
| Suggested primer 2: | CTCTTTGTTTTGGAGGCAGC |