Insertion junction: LMJ.RY0402.119603_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.119603
Chromosome:chromosome 1
Location:4471988
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre01.g030450 (1 of 1) IPR006615//IPR028881//IPR028889//IPR029071 - Peptidase C19, ubiquitin-specific peptidase, DUSP domain // PAN2 domain // Ubiquitin specific protease domain // Ubiquitin-related domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGCGGAGGAGGTGCGGCGGCGCGTCGCG
Internal bar code:TCGAGGCGGCGATGTAGAAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:143
LEAP-Seq percent confirming:45.31
LEAP-Seq n confirming:285
LEAP-Seq n nonconfirming:344
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CTAGAGGTGCTCTTGTCCCG
Suggested primer 2:TCATCCCCTGTCAAATCCAT