| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119610 |
| Chromosome: | chromosome 16 |
| Location: | 6080874 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676050 | ARP3 | (1 of 1) K18584 - actin-related protein 3 (ACTR3, ARP3); Actin-related protein | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAAGGACTTGACCGTTGCAGTGATTGAC |
| Internal bar code: | ATTAGGCGGACATTAACAAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 774 |
| LEAP-Seq percent confirming: | 98.4713 |
| LEAP-Seq n confirming: | 9340 |
| LEAP-Seq n nonconfirming: | 145 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCTCCTGCTCCTCCTCCTC |
| Suggested primer 2: | TACACGCTCACAGTTACGGC |