| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.119616 |
| Chromosome: | chromosome 12 |
| Location: | 8396671 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548900 | ROC23,ROC112 | (1 of 2) PTHR23181//PTHR23181:SF7 - RHO GTPASE ACTIVATING PROTEIN // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGTGGACTGTCTTGTGGGTTTAAGTGTG |
| Internal bar code: | GCCAACGAGTTCCCCTATACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 432 |
| LEAP-Seq percent confirming: | 99.6967 |
| LEAP-Seq n confirming: | 1315 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTCCTAGCGTCGGATGATG |
| Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |