| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119645 |
| Chromosome: | chromosome 2 |
| Location: | 6820632 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119100 | CER2 | (1 of 2) K15889 - prenylcysteine alpha-carboxyl methylesterase (PCME); Carboxyesterase-related protein/arylformamidase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCCATTATCACCTCGTCTGAACCGGTG |
| Internal bar code: | CCGCTCCGGTCCACGCGCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 99.6183 |
| LEAP-Seq n confirming: | 261 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGCCAACGAGTAGTGTCG |
| Suggested primer 2: | CACATACACACCTTGTCGCC |