Insertion junction: LMJ.RY0402.119650_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.119650
Chromosome:chromosome 7
Location:1176362
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g320800 (1 of 1) PF09468 - Ydr279p protein family (RNase H2 complex component) (RNase_H2-Ydr279) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACGCTGCACACGATAATAAAATGCCAAA
Internal bar code:TCCGATCAAACCCCTGAGTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:657
LEAP-Seq percent confirming:99.2813
LEAP-Seq n confirming:967
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTGTAATGTGGTGCGATG
Suggested primer 2:GTCGTTCAGGCGGTAGTAGC