| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119675 |
| Chromosome: | chromosome 7 |
| Location: | 2138194 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g326550 | (1 of 12) IPR008928 - Six-hairpin glycosidase-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATGCATTTACTGCACGCGGATGACGGCGG |
| Internal bar code: | GCCCACCGGTTGGGAATCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 97.9862 |
| LEAP-Seq n confirming: | 3260 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTGTTGGCTTTCCTGCTC |
| Suggested primer 2: | TGTTCTGCCACCAGCAGAT |