Insertion junction: LMJ.RY0402.119679_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.119679
Chromosome:chromosome 2
Location:6077788
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g112900 TPT6,TPT5 (1 of 3) K15356 - GDP-mannose transporter (VRG4, GONST1); Putative GDP-mannose transporter 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGAACAGAGGTCTAGGTAACAAGTAGGA
Internal bar code:ACTGCCGACAATAGTGTAATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:889
LEAP-Seq percent confirming:99.4186
LEAP-Seq n confirming:13167
LEAP-Seq n nonconfirming:77
LEAP-Seq n unique pos:84

Suggested primers for confirmation by PCR

Suggested primer 1:GCAAGGGAAGGAGGATAAGG
Suggested primer 2:CTTGTTGTGCGTGGACACTT