| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.119693 |
| Chromosome: | chromosome 16 |
| Location: | 6024298 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676402 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTTCCTGTCTGTCCATGCAGCCACGCA |
| Internal bar code: | CACGTGCTCCTCTCTGCTAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 77.2776 |
| LEAP-Seq n confirming: | 10747 |
| LEAP-Seq n nonconfirming: | 3160 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGTGTGCAACTAGGACCG |
| Suggested primer 2: | ACCCGGCAAATGAACTGTAG |