| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119712 |
| Chromosome: | chromosome 8 |
| Location: | 3608768 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g376000 | (1 of 1) PTHR12906:SF0 - PROTEIN F44E7.9 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGCGACCGTGTGCAGCAAGCAGTGTAC |
| Internal bar code: | AGCCCCGCGAGAGACGGTTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 882 |
| LEAP-Seq percent confirming: | 98.1164 |
| LEAP-Seq n confirming: | 8230 |
| LEAP-Seq n nonconfirming: | 158 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATACACTCCCCACGCTTC |
| Suggested primer 2: | CGGGACTGAGTACAGGTGGT |