| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.119715 |
| Chromosome: | chromosome 17 |
| Location: | 5159081 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g735450 | SUB14 | (1 of 14) 3.4.21.62 - Subtilisin; Subtilisin-like protease | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCTGCGGGAGACGGGCGGGCTCATGCG |
| Internal bar code: | GCCGGCGCGCTACTCCGTCCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 623 |
| LEAP-Seq percent confirming: | 98.9565 |
| LEAP-Seq n confirming: | 569 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGATGGGGAGTTCATTG |
| Suggested primer 2: | ACCAGCAGACCCAGACAGAT |