| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119752 |
| Chromosome: | chromosome_2 |
| Location: | 1086931 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g081050 | FAP24 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCATTGTCCCCTGTCTCTCGATTTTGATGA |
| Internal bar code: | GTCACCGTGGCCAGTCGAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 938 |
| LEAP-Seq percent confirming: | 98.722 |
| LEAP-Seq n confirming: | 10506 |
| LEAP-Seq n nonconfirming: | 136 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATGCAATGCCGTGTTGTT |
| Suggested primer 2: | CAACCTAGCCTCACAGCACA |