| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.119779 |
| Chromosome: | chromosome 9 |
| Location: | 4097406 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g393580 | (1 of 2) PF03097 - BRO1-like domain (BRO1) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGCCAGTCCGCCCAGATGGCCTGCGGGC |
| Internal bar code: | TCCCTCCCGCGAATTGCAATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 467 |
| LEAP-Seq percent confirming: | 93.2432 |
| LEAP-Seq n confirming: | 138 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCACAGGCCACTGTAAAA |
| Suggested primer 2: | ACACACACACACACAGGGCT |