| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.119817 |
| Chromosome: | chromosome 8 |
| Location: | 143780 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g358539 | (1 of 2) PTHR13009:SF8 - AHSA1 PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTTGAAAGGGGAAGTTAGCGGGACGCGG |
| Internal bar code: | GCGATTCGAGGTGGAACATGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 165 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACGCATAACCGCCAATA |
| Suggested primer 2: | ACCCCTCCACTATTCATCCC |