| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119846 |
| Chromosome: | chromosome 3 |
| Location: | 2723462 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161550 | FAP55 | (1 of 1) IPR009053//IPR023220 - Prefoldin // Type IV secretion system, VirB5-domain; Flagellar Associated Protein 55 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCGGACCGGCTGGGCGGCAAGGTGAGG |
| Internal bar code: | GGACGTGCAACGCACTGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 388 |
| LEAP-Seq percent confirming: | 99.6604 |
| LEAP-Seq n confirming: | 1174 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCACAGGATGACCTGGAT |
| Suggested primer 2: | AGGCGTAAGCAACAGCATTT |