| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119894 |
| Chromosome: | chromosome 16 |
| Location: | 417711 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g693300 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTCGCCAGTATGGATGGTATTCACCATAT |
| Internal bar code: | GGCCCCCGGGGTAGACACCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 763 |
| LEAP-Seq percent confirming: | 99.7579 |
| LEAP-Seq n confirming: | 2060 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCAGTGTAGGCGAAAGG |
| Suggested primer 2: | GTGGGGACAGCTAATGCCTA |