| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.119896 |
| Chromosome: | chromosome 8 |
| Location: | 3887400 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378050 | NUOP5 | NADH:ubiquinone oxidoreductase 16 kDa subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAAGCACGCGCCGCGGTGGTGGTGGCCA |
| Internal bar code: | ACAGTTACACGATCTCGGTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 853 |
| LEAP-Seq percent confirming: | 96.1696 |
| LEAP-Seq n confirming: | 703 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAACGTCTTCAGGTTGT |
| Suggested primer 2: | TGCAGCAGCCCTAAAAATCT |