| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.119946 |
| Chromosome: | chromosome 6 |
| Location: | 6072654 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g289700 | BET5 | (1 of 1) PTHR23249//PTHR23249:SF16 - SYNBINDIN // TRAFFICKING PROTEIN PARTICLE COMPLEX SUBUNIT 1; Component of TRAPP complex | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGCTGGACGACTCAAGGGCCGCCATCT |
| Internal bar code: | GGTCAAACGCTCTGTATGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 64 |
| LEAP-Seq percent confirming: | 98.419 |
| LEAP-Seq n confirming: | 249 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGAGCTTCTCGCTGTGGT |
| Suggested primer 2: | TCTTGATGAGGCAACTGCAC |