| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.119954 |
| Chromosome: | chromosome_15 |
| Location: | 1281255 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre15.g641650 | HLM22 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGAACGGCAGCTCGTCATCGTAACCGTCAC |
| Internal bar code: | TTGCTCTGGGAAACCGGTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 85 |
| LEAP-Seq percent confirming: | 97.2222 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGTCCCTCTGTCTTGCG |
| Suggested primer 2: | TGCAGAGGCAGTACAGGATG |