| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.120025 |
| Chromosome: | chromosome 2 |
| Location: | 5499277 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g108200 | CGL80 | Conserved in the Green Lineage; (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAGAATGGGCGGCACAACCCGCTTCGCTA |
| Internal bar code: | CGAAGGGTCGAGGAAGTCGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1126 |
| LEAP-Seq percent confirming: | 99.4496 |
| LEAP-Seq n confirming: | 7950 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTAGTGCTGATCGTGAAA |
| Suggested primer 2: | GAAATGGAAAGGCTTAGGGC |