| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.120040 |
| Chromosome: | chromosome 6 |
| Location: | 6544280 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAAGTAAGGACGTTTTGACCTTTCAGGT |
| Internal bar code: | TACCGATGTTGCGGATGGCCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 844 |
| LEAP-Seq percent confirming: | 82.6087 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCTCTTTCTTCTTGCG |
| Suggested primer 2: | ACGAGCACGACAAACAACAG |