| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.120049 |
| Chromosome: | scaffold_18 |
| Location: | 241391 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre18.g749947 | FAP21 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CGTGCAAACAGGTCAGTCATCGAGCACGTG |
| Internal bar code: | GAGCACTCGGTTATCCCGCCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 183 |
| LEAP-Seq percent confirming: | 99.5924 |
| LEAP-Seq n confirming: | 2932 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTCAACACAGACGCATT |
| Suggested primer 2: | GCACTTGTTATTCGGTCCGT |