| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.120056 |
| Chromosome: | chromosome 16 |
| Location: | 5285803 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682552 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGCACCGAAACTACCAAGCAAGCTCAAT |
| Internal bar code: | TTTTATTTAATGGAGAGTTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 776 |
| LEAP-Seq percent confirming: | 99.7732 |
| LEAP-Seq n confirming: | 440 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGACGGTGTCAGCAGTG |
| Suggested primer 2: | GCATAAGGCTCAGTTAGCGG |