| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120073 |
| Chromosome: | chromosome 6 |
| Location: | 7154971 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g297650 | UBC36 | (1 of 1) K10582 - ubiquitin-conjugating enzyme E2 Q (UBE2Q); E2 ubiquitin conjugating enzyme | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTACGAATCGGCGTGGAATGGGGCGTT |
| Internal bar code: | TTTGTCCGTGGCAGTAAGCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 283 |
| LEAP-Seq percent confirming: | 86.6197 |
| LEAP-Seq n confirming: | 123 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCTTCAATTTGCGCTTT |
| Suggested primer 2: | CCAAAATCCTTGTCCTCCAA |