Insertion junction: LMJ.RY0402.120102_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.120102
Chromosome:chromosome 7
Location:2168815
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g326750 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGGCTCAGTTGTGGCCGTACGCCGGATGC
Internal bar code:GCCTCTCCCGTAACTCCGAGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:553
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:255
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGAAGCTGACCCTGAGGTAG
Suggested primer 2:TACTGAAACAACGACTGCCG