| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120116 |
| Chromosome: | chromosome 2 |
| Location: | 1645009 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g085400 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTTTGATTCGCGTGTCCAAAGAGGCGCC |
| Internal bar code: | CTCGTCCTGCACGCAAACAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 300 |
| LEAP-Seq percent confirming: | 96.2181 |
| LEAP-Seq n confirming: | 1094 |
| LEAP-Seq n nonconfirming: | 43 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATAAGCGCTTCTTTACGGC |
| Suggested primer 2: | GACGCCAGAGCCAGAGATAC |