| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.120137 |
| Chromosome: | chromosome 1 |
| Location: | 4742526 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032800 | MRS2,MRS6 | Mg2+ transporter protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGATGCGCACGTGCTCCATCGCGAAGA |
| Internal bar code: | ATACGCGGCCGGTTAGATCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 671 |
| LEAP-Seq percent confirming: | 99.7591 |
| LEAP-Seq n confirming: | 4970 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCATGTGTGCGATAAAGC |
| Suggested primer 2: | GTCTCCCTTCCACTTCCCTC |