| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.120138 |
| Chromosome: | chromosome 6 |
| Location: | 6836436 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295150 | (1 of 4) PF13515 - Fusaric acid resistance protein-like (FUSC_2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACCCCCCTCATCGCTCTTCACTCCCCAT |
| Internal bar code: | ATGGTGTCTATTGCAGTTTTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 158 |
| LEAP-Seq percent confirming: | 99.6628 |
| LEAP-Seq n confirming: | 2069 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTTTGTGTATTGCGGG |
| Suggested primer 2: | CAGCAGGTGTAGCCCTCTTC |