| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120149 |
| Chromosome: | chromosome 7 |
| Location: | 2221236 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g327250 | (1 of 3) IPR000104//IPR005135 - Antifreeze protein, type I // Endonuclease/exonuclease/phosphatase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGAGTAAATGGCTGATTAGTCTATAAA |
| Internal bar code: | TCGGAGGTGGTCCTGACCACTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 269 |
| LEAP-Seq percent confirming: | 94.6809 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAAGGAAGCTGGAGATTT |
| Suggested primer 2: | AAGCTTCTTCGCACACCAAT |