| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120155 |
| Chromosome: | chromosome 11 |
| Location: | 223273 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467552 | (1 of 3) K00850 - 6-phosphofructokinase 1 (pfkA, PFK) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCGACCGCACCCCTGGGGAGATGTTCT |
| Internal bar code: | TTTGGATGATCTAGGTAGAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 582 |
| LEAP-Seq percent confirming: | 99.9129 |
| LEAP-Seq n confirming: | 2293 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGACTCCACTGCTGCTG |
| Suggested primer 2: | TCCCACCTAGACGTTTGTCC |