Insertion junction: LMJ.RY0402.120169_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.120169
Chromosome:chromosome 14
Location:3919952
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre14.g632900 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCCGGGCGCGGGCGGCGGAGCAGGAGC
Internal bar code:CCGGCATAAGACCGTCGGTTGT

Confirmation - LEAP-Seq

LEAP-Seq distance:391
LEAP-Seq percent confirming:33.6932
LEAP-Seq n confirming:593
LEAP-Seq n nonconfirming:1167
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTGCACACACACACACAC
Suggested primer 2:ACCTGCAGTTCAACGTACCC