| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120169 |
| Chromosome: | chromosome 14 |
| Location: | 3920050 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g632900 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCGCTCTGACCTCGCCTCCTCCTCCTCC |
| Internal bar code: | GCAACGGCGCTTATGATAACCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 83 |
| LEAP-Seq percent confirming: | 10.6195 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 505 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCTGCAGTTCAACGTACCC |
| Suggested primer 2: | CCACTCACCACACACACACA |