| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.120175 |
| Chromosome: | chromosome 7 |
| Location: | 1647820 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325300 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGATGTCCCAAGGCCCTTGGCCTCCCC |
| Internal bar code: | AATCCTTGGCATTATTACCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 561 |
| LEAP-Seq percent confirming: | 57.992 |
| LEAP-Seq n confirming: | 4205 |
| LEAP-Seq n nonconfirming: | 3046 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCGACATAACTCTTGCG |
| Suggested primer 2: | CCAGCAGCTGTAGCAATCAA |