| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120199 |
| Chromosome: | chromosome 7 |
| Location: | 677549 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g317350 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGCGCTGCACCACGGCCGCGCCATCAG |
| Internal bar code: | AACTATATCCGAGGTAGGACCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 596 |
| LEAP-Seq percent confirming: | 98.8818 |
| LEAP-Seq n confirming: | 619 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGTCAATAATCGGTGTCG |
| Suggested primer 2: | AAATTGTTGGTTCAGGCGAC |