Insertion junction: LMJ.RY0402.120202_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.120202
Chromosome:chromosome 7
Location:5882475
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre07.g353837 (1 of 80) IPR003882 - Pistil-specific extensin-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTCTCCTCCTCCGTCGCCTCCTCCTCCG
Internal bar code:TTCTTTCCTCTCATAGTGGACT

Confirmation - LEAP-Seq

LEAP-Seq distance:15
LEAP-Seq percent confirming:93.3333
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTTAAGGCATGTGCTTG
Suggested primer 2:GCACTCTACGCACACCTTGA