| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120223 |
| Chromosome: | chromosome 10 |
| Location: | 6262723 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g464900 | (1 of 10) 3.1.1.5 - Lysophospholipase / Phospholipase B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGATGGGAGCATGCGGTGTGGTGGTAGTT |
| Internal bar code: | GACGAATACGTCGGCCCGGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 319 |
| LEAP-Seq percent confirming: | 99.226 |
| LEAP-Seq n confirming: | 3205 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTTGTAGGAGGGCCAGTG |
| Suggested primer 2: | CCATGCTGCGACTCTACAAA |