| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.120264 |
| Chromosome: | chromosome 6 |
| Location: | 4097270 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278219 | SSA6 | cilia-sensing, structure and/or assembly; (1 of 239) IPR016024 - Armadillo-type fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTCAGGTGCGTGCGTACGACGCATTCGGG |
| Internal bar code: | GAAGTATGTTTGTTTCAGGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 383 |
| LEAP-Seq percent confirming: | 99.391 |
| LEAP-Seq n confirming: | 1632 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACAGAAAGAAGGCGATGC |
| Suggested primer 2: | AGCCCAAACTAAAGAGGGGA |