| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.120352 |
| Chromosome: | chromosome 14 |
| Location: | 564932 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g611750 | (1 of 24) IPR001680//IPR017986//IPR020472 - WD40 repeat // WD40-repeat-containing domain // G-protein beta WD-40 repeat | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGGAGGCGGTGAGAGTCCATTCAGTCA |
| Internal bar code: | GCTAAGAGGTGCCCTGGTCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 794 |
| LEAP-Seq percent confirming: | 96.6667 |
| LEAP-Seq n confirming: | 1015 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGTCTGGATGTTGGGTA |
| Suggested primer 2: | CTACCCCAACCCATCACATC |