| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.120398 |
| Chromosome: | chromosome 10 |
| Location: | 640110 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g422000 | TMG6,TRM1 | (1 of 1) PTHR10631:SF3 - TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE; tRNA (guanine-N2)-methyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAACGTTGATAATATTTGAGTCAGGCTG |
| Internal bar code: | CTTCGGCTACCGTTCCAGTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 601 |
| LEAP-Seq percent confirming: | 99.6033 |
| LEAP-Seq n confirming: | 4519 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGGTTTAGAAGGGTGGC |
| Suggested primer 2: | GCTCTCGCACTCCTGATTTC |