| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.120403 |
| Chromosome: | chromosome 14 |
| Location: | 2292937 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g623350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCATATAATGGCAACGGCCAACGGGGACA |
| Internal bar code: | GCGTGGGGTGAGCCGTACGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 98.4593 |
| LEAP-Seq n confirming: | 17510 |
| LEAP-Seq n nonconfirming: | 274 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACAGGGAGCCGGTGTTTA |
| Suggested primer 2: | TACACAAGGGCTACAAGGGC |