| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.120439 |
| Chromosome: | chromosome 4 |
| Location: | 2347840 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220825 | (1 of 3) PF10601 - LITAF-like zinc ribbon domain (zf-LITAF-like) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATGGCACACGCTGACACTGCCGGCAAC |
| Internal bar code: | AGCGCGACAGGTCATTATACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1066 |
| LEAP-Seq percent confirming: | 99.4955 |
| LEAP-Seq n confirming: | 986 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACACGAGGGACCTGCAT |
| Suggested primer 2: | TCGTGGAAGCATGAAGACAG |