| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.120452 |
| Chromosome: | chromosome 16 |
| Location: | 5313506 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682305 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTTGAGGACGGCTGTGCGGGACCTGACG |
| Internal bar code: | GATCGGAGTCGACCGGGTAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 92.2607 |
| LEAP-Seq n confirming: | 906 |
| LEAP-Seq n nonconfirming: | 76 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGCCACCGTATGTCAAGTC |
| Suggested primer 2: | AGACACGGAGCTCAGCAAAT |