| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.120488 |
| Chromosome: | chromosome 12 |
| Location: | 9439602 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g539750 | (1 of 1) PF09649 - Histone chaperone domain CHZ (CHZ) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCTGCACCACGAGGACGGGGGGACAGCG |
| Internal bar code: | CTAAACGGACAATCGAGTAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 720 |
| LEAP-Seq percent confirming: | 90.7407 |
| LEAP-Seq n confirming: | 392 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTCCCGCTTTGTTACTG |
| Suggested primer 2: | AAAGACCAAGACATCACCGC |