| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.120505 |
| Chromosome: | chromosome 7 |
| Location: | 1948296 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325749 | (1 of 1) PF14924 - Protein of unknown function (DUF4497) (DUF4497) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCGGAGCTCTTAGCGCTGTTTGGAACT |
| Internal bar code: | ATCCTGGCAAGCAGCCCCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 578 |
| LEAP-Seq percent confirming: | 90.2521 |
| LEAP-Seq n confirming: | 1074 |
| LEAP-Seq n nonconfirming: | 116 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCGTGAGTACCGTATTG |
| Suggested primer 2: | TCTTGTCTTGTTCAGGCACG |