| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.120510 |
| Chromosome: | chromosome 13 |
| Location: | 3765302 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g589600 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATGAGCTCAGCTCCTCCCAGCTCCCAAC |
| Internal bar code: | GGCATAGGCAACGTCCGGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 239 |
| LEAP-Seq percent confirming: | 99.8214 |
| LEAP-Seq n confirming: | 559 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTATTCTGGTGTGGGTCGCT |
| Suggested primer 2: | GATTGCAAGAGGTGGTGTGA |