| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.120536 |
| Chromosome: | chromosome 2 |
| Location: | 526399 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077100 | GSH1 | (1 of 1) 6.3.2.2 - Glutamate--cysteine ligase / Gamma-glutamylcysteine synthetase; Gamma-glutamylcysteine synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTCAAGTCACTTAACGTCGTCTTTCCGAC |
| Internal bar code: | CTTATCACGGGCGAGTAACGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 728 |
| LEAP-Seq percent confirming: | 64.8863 |
| LEAP-Seq n confirming: | 2539 |
| LEAP-Seq n nonconfirming: | 1374 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCGCAGGAGTTGTATTGTT |
| Suggested primer 2: | TAGTGCATACTGCCTTTGCG |